A detailed biostratigraphic study, based on calcareous nannofossils, was performed to re-evaluate the stratigraphic interpretation of the Numidian Formation and its structural relationship with the underlying El Haria, Boudabbous and Souar formations Tellian units (i.e., stratigraphic vs. tectonic). The new biostratigraphic results indicate that the Numidian Formation is not restricted to a Miocene age as considered in some recent works but spans from Oligocene (Rupelian and Chattian, NP23 to NP25) to early Miocene (Aquitanian and Burdigalian, NN1 to NN3). The Cap Serrat succession can be retained as the type section for the Tunisian Numidian Formation, where both the Chattian-Aquitanian (Oligocene/Miocene boundary) and the Aquitanian-Burdigalian transitions have been characterized. Based on the new calcareous nannofossil findings, several duplications of the sedimentary succession were identified. They include the thick shales and marls of El Haria (Maastrichtian-Paleocene) and Souar (Eocene) formations, thrusted by the Numidian Formation. The updated regional structural cross-sections, confirm that the Numidian Formation is currently juxtaposed by southward major thrust contact to the underlying Kasseb structural unit in the studied area. The latter is intensively affected by poly-phased tectonics, resulting in the thrust events associated with the Alpine phase inversion. This has to be taken into consideration in oil and gas exploration in the area of the fold-thrust belt system, in both onshore and offshore Northern Tunisia.